Search results for "Laser Excitation"
showing 9 items of 9 documents
Precision and accuracy of single-molecule FRET measurements-a multi-laboratory benchmark study
2018
Single-molecule Forster resonance energy transfer (smFRET) is increasingly being used to determine distances, structures, and dynamics of biomolecules in vitro and in vivo. However, generalized protocols and FRET standards to ensure the reproducibility and accuracy of measurements of FRET efficiencies are currently lacking. Here we report the results of a comparative blind study in which 20 labs determined the FRET efficiencies (E) of several dye-labeled DNA duplexes. Using a unified, straightforward method, we obtained FRET efficiencies with s.d. between +/- 0.02 and +/- 0.05. We suggest experimental and computational procedures for converting FRET efficiencies into accurate distances, and…
FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices.
2021
International audience; Single-molecule FRET (smFRET) has become a mainstream technique for studying biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by an ever- increasing number of groups has generated significant progress in sample preparation, measurement procedures, data analysis, algorithms and documentation. Several labs that employ smFRET approaches have joined forces to inform the smFRET community about streamlining how to perform experiments and analyze results for obtaining quantitative information on biomolecular structure and dynamics. The recent efforts include blind tests to assess the accuracy and the precision of smFRET experiments among d…
Radiation hardening of Rare-Earth doped fiber amplifiers
2012
We investigated the radiation hardening of optical fiber amplifiers operating in space environments. Through a real-time analysis in active configuration, we evaluated the role of Ce in the improvement of the amplifier performance against ionizing radiations. Ce-codoping is an efficient hardening solution, acting both in the limitation of defects in the host glass matrix of RE-doped optical fibers and in the stabilization of lasing properties of the Er3+-ions. On the one hand, in the nearinfrared region, radiation induced attenuation measurements show the absence of radiation induced P-related defect species in host glass matrix of the Ce-codoped active fibers; on the other hand, in the Ce-…
Unexpected photochemistry and charge-transfer complexes of [CB11H12]2 carborane
2008
Although the [CB11H12]¯carborane does not exhibit an absorption band inUV, its triplet excited state can be generated upon 308 nm laser excitation; also unexpectedly carborane acts as electron donor forming a charge transfer complex with methylviologen that upon illumination gives rise to viologen radical cation. Galletero Pedroche, M.Sales, Sales.Galletero@uv.es
Internal conversion from excited electronic states of 229Th ions
2017
The process of internal conversion from excited electronic states is investigated theoretically for the case of the vacuum-ultraviolet nuclear transition of 229Th. Due to the very low transition energy, the 229Th nucleus offers the unique possibility to open the otherwise forbidden internal conversion nuclear decay channel for thorium ions via optical laser excitation of the electronic shell. We show that this feature can be exploited to investigate the isomeric state properties via observation of internal conversion from excited electronic configurations of Th+ and Th2+ ions. A possible experimental realization of the proposed scenario at the nuclear laser spectroscopy facility IGISOL in J…
Role of Ce3+ as sensitizer for the infrared luminescence of phosphosilicate Er/Yb doped glasses
2012
The luminescence properties of the Yb/Er-doped phosphosilicate preforms used for the design of active optical fibers were investigated under a tunable laser excitation from ultraviolet to infrared domain. We demonstrated that codoping the glass matrix with Ce3+ ions strongly influences the infrared emission associated with Er3+ ions, it enhances the energy transfer from Yb3+ to Er3+ ions, and it provides an additional ultraviolet excitation channel for the emission of both Yb3+ and Er3+ ions. The excitation/emission pathways are discussed on the basis of models proposed in literature for other systems.
Modèles quantiques à deux états avec croisements de niveaux décrits par les fonctions de Heun
2019
The thesis is devoted to the fundamental problem of excitation and manipulation of quantum systems, having discrete energy spectrum, via external laser fields. We examine the semiclassical time- dependent quantum two-state problem, when the external electromagnetic field is resonant or quasi-resonant for some two of many levels of the system. The focus of the thesis is on the analytic description of the non- adiabatic evolution of quantum systems subject to excitation by level-crossing field configurations. In the present thesis we classify the complete set of the semiclassical time-dependent quantum two-state models solvable in terms of the five function of the Heun class.Main results of t…
All-Fiber Laser With Intracavity Acousto-Optic Dynamic Mode Converter for Efficient Generation of Radially Polarized Cylindrical Vector Beams
2017
We report an all-fiber laser that emits a radially polarized cylindrical vector beam (CVB) at 1 μm based on an intracavity acousto-optic mode converter. We efficiently generate the CVB, taking advantage of the acousto-optic coupling from the HE11 mode to the TM01 mode in a two-mode fiber. The laser can be switched from emitting a Gaussian-like beam to a radially polarized CVB. Radially polarized CVBs with modal and polarization purities >98% and a maximum power of 65 mW were generated.
Internal conversion from excited electronic states of 229Th ions
2017
The process of internal conversion from excited electronic states is investigated theoretically for the case of the vacuum-ultraviolet nuclear transition of 229 Th . Due to the very low transition energy, the 229 Th nucleus offers the unique possibility to open the otherwise forbidden internal conversion nuclear decay channel for thorium ions via optical laser excitation of the electronic shell. We show that this feature can be exploited to investigate the isomeric state properties via observation of internal conversion from excited electronic configurations of Th + and Th 2 + ions. A possible experimental realization of the proposed scenario at the nuclear laser spectroscopy facility IGISO…